Wolverine Peptide Blend Reconstitution Calculator

Wolverine is a research peptide blend supplied as a single lyophilized vial combining BPC-157 and TB-500 at an even ratio. Because both peptides ship premixed, reconstituting the vial is a one-time step that sets the concentration for the whole blend at once. This reference walks through that process and how to use the peptide reconstitution calculator to convert a Wolverine vial and target dose into syringe units. It is written for research purposes only and is not medical advice.

What the calculator does

The peptide reconstitution calculator handles the math in two directions:

• Forward mode — enter the vial's total mg, water volume, and target dose → it returns the syringe units to draw.
• Reverse mode — enter the vial's total mg and target dose, plus the units you want to land on → it tells you how much water to add.

Both modes work from the same two-step formula: concentration equals total mass divided by water volume, and units drawn equal volume in mL multiplied by 100 on a U-100 insulin syringe. For a Wolverine vial, "total mg" means the combined mass of both peptides, since a single draw pulls both out together.

Wolverine vial composition

A Wolverine vial is commonly supplied at an even 10 mg / 10 mg split:

Peptide	Amount
BPC-157	10 mg
TB-500	10 mg
Total	20 mg

That's a 1:1 ratio between the two. Reconstituting the vial dissolves both into one solution — there's no way to draw one component on its own from a premixed vial. Some suppliers also sell a larger 40 mg vial (20 mg of each peptide) that holds the same ratio at double the total mass.

Typical reconstitution setup

A common research setup for a 20 mg Wolverine vial uses 2 mL of bacteriostatic water:

Vial content	Water added	Concentration
20 mg (10/10 Wolverine)	2 mL	10 mg/mL (10,000 mcg/mL)

More water lowers the concentration and produces a larger, easier-to-read draw. Less water raises the concentration and shrinks the draw. Enter a different vial total or water volume into the calculator if your Wolverine vial isn't the 20 mg standard — a 40 mg vial, for instance, needs proportionally more water to land at the same concentration.

Reconstitution process

1. Gather supplies — the Wolverine vial, bacteriostatic water, U-100 insulin syringes, alcohol swabs.
2. Swab both vial stoppers with alcohol.
3. Draw the calculated water volume (e.g. 2 mL for a 20 mg vial).
4. Inject slowly down the inside wall of the vial, not directly onto the powder.
5. Swirl gently — do not shake. Let it dissolve completely.
6. Verify the concentration matches what you entered in the calculator before drawing a dose.

For the general reconstitution steps that apply to any peptide vial, see how to reconstitute peptides.

The math

Step 1: Calculate concentration

Concentration = total vial mass ÷ water volume

20 mg ÷ 2 mL = 10 mg/mL, or 10,000 mcg/mL (1 mg = 1,000 mcg)

Step 2: Convert dose to volume

Volume (mL) = target dose (mcg) ÷ concentration (mcg/mL)

Example: 300 mcg ÷ 10,000 mcg/mL = 0.03 mL

Step 3: Convert volume to syringe units

On a U-100 insulin syringe, 1 mL equals 100 units, so:

Units = volume (mL) × 100

Example: 0.03 mL × 100 = 3 units

The calculator performs these three steps instantly for any vial total, water volume, and dose you enter.

Worked example

A 20 mg Wolverine vial (10 mg BPC-157, 10 mg TB-500) reconstituted with 2 mL of bacteriostatic water sits at 10,000 mcg/mL. A 300 mcg total dose from that vial is 3 units on a U-100 syringe. Because the vial holds its two peptides at a 1:1 ratio, that 300 mcg draw splits evenly into 150 mcg of BPC-157 and 150 mcg of TB-500.

If 3 units is awkward to read on your syringe, reverse mode solves for a rounder mark instead. Enter the 20 mg vial total, the same 300 mcg dose, and a target of 6 units, and the calculator returns 4 mL of water rather than 2 mL. Doubling the water halves the concentration to 5,000 mcg/mL, which doubles the volume — and the unit reading — needed to deliver the same 300 mcg dose.

Storage

Reconstituted Wolverine vials are generally kept refrigerated and protected from light, the same as any reconstituted peptide solution. Unreconstituted lyophilized powder is more stable than the mixed solution, which is why the vial ships dry. Track the date you added water so you know how long a given vial has been mixed.

Common mistakes

• Treating the vial's total mg as a single peptide's dose. A "300 mcg Wolverine dose" is 300 mcg of combined peptide across two compounds, not 300 mcg of each.
• Reusing a water volume from a single-peptide guide. BPC-157 alone and a Wolverine vial hold different total mg, so the same water volume produces a different concentration in each.
• Mixing up mg and mcg. The vial label reads in milligrams, but Wolverine doses are typically referenced in micrograms, and 1 mg equals 1,000 mcg.
• Shaking instead of swirling, which can degrade the peptides.
• Losing track of which vial is which concentration if a Wolverine vial sits in the fridge next to single-peptide vials. Label each one.

Use the calculator

Enter your Wolverine vial's total mg, bacteriostatic water volume, and target dose into the free peptide calculator to see the exact syringe units, or switch to reverse mode to find the water volume that lands a dose on a specific unit mark. For the component-level breakdown of a Wolverine dose across BPC-157 and TB-500, see the Wolverine peptide blend dosage guide. For vial-size options and how they affect total supply, see the Wolverine peptide blend vial size guide.