Wolverine Peptide Blend Dosage Guide: Reconstitution & Units

Wolverine is a common name for a research peptide blend that combines BPC-157 and TB-500 in a single vial, at an even 1:1 ratio. Because the two peptides ship premixed, a Wolverine peptide blend dosage guide has to account for one extra step that single-peptide guides don't: the vial's total milligram count is split across two compounds in a fixed ratio, and that ratio carries through every draw. This is a research reference for that math, not medical advice.

What's in a Wolverine peptide blend

A Wolverine vial is typically supplied as lyophilized powder containing two peptides at equal amounts:

• BPC-157 — 10 mg
• TB-500 — 10 mg

That's 20 mg of total peptide content per vial, split evenly at a 1:1 ratio. Reconstitution dissolves both into a single solution — there's no way to draw one peptide without the other, since they're mixed together before the water goes in.

Typical reconstitution setup

BPC-157 and TB-500 are each usually reconstituted with enough water to land their typical mcg-scale doses on clean syringe marks, and a combined Wolverine vial follows the same logic. A 2 mL water volume on a 20 mg (10/10) vial is a common reference point:

Vial content	Water added	Concentration
20 mg (10/10 Wolverine)	2 mL	10 mg/mL (10,000 mcg/mL)

More water lowers the concentration and produces a larger, easier-to-read draw. Less water raises the concentration and shrinks the draw. The ratio between BPC-157 and TB-500 stays 1:1 no matter how much water you add — water changes concentration, not composition.

The math

Step 1: Calculate total concentration

Concentration = total vial mass ÷ water volume

20 mg ÷ 2 mL = 10 mg/mL, or 10,000 mcg/mL (1 mg = 1,000 mcg)

Step 2: Convert dose to volume

Volume (mL) = target dose (mcg) ÷ concentration (mcg/mL)

Example: 500 mcg ÷ 10,000 mcg/mL = 0.05 mL

Step 3: Convert volume to syringe units

On a U-100 insulin syringe, 1 mL equals 100 units, so:

Units = volume (mL) × 100

Example: 0.05 mL × 100 = 5 units

Worked example

Take a 20 mg Wolverine vial (10 mg BPC-157, 10 mg TB-500) reconstituted with 2 mL of bacteriostatic water, for a concentration of 10 mg/mL, or 10,000 mcg/mL.

A 500 mcg total blend dose comes out to 5 units on a U-100 syringe. Because the vial holds the two peptides at a 1:1 ratio, that same 500 mcg draw splits evenly:

• BPC-157: 500 mcg × (10 ÷ 20) = 250 mcg
• TB-500: 500 mcg × (10 ÷ 20) = 250 mcg

Those two figures add back up to the 500 mcg total dose. The ratio holds at any dose size — a 250 mcg total dose splits into 125 mcg of each, and a 1,000 mcg (1 mg) dose splits into 500 mcg of each.

Dose chart

Target total dose	Volume drawn	Units (U-100)
100 mcg	0.01 mL	1 unit
250 mcg	0.025 mL	2.5 units
500 mcg	0.05 mL	5 units
750 mcg	0.075 mL	7.5 units
1,000 mcg (1 mg)	0.1 mL	10 units

Rather than working through this by hand, enter the vial's total mg, water volume, and target dose into the peptide dosage calculator and it returns the exact units, including a reverse mode that solves for the water volume needed to land a dose on a cleaner mark.

Wolverine versus GLOW

Wolverine shares two of its components with the GLOW blend, but the compositions diverge from there. Wolverine is BPC-157 and TB-500 only, 10 mg each, for a 20 mg vial at a 1:1 ratio — the same two peptides GLOW includes, without GLOW's added 50 mg of GHK-Cu. That absence changes how each blend gets dosed: GLOW's GHK-Cu share pushes its typical total dose into the milligram range, while Wolverine's smaller, mcg-scale components keep its typical doses in the hundreds of micrograms, the same range BPC-157 and TB-500 use individually. Mixing up which vial is which throws off every draw calculated from it, so treat Wolverine and GLOW as separate entries even where the reconstitution steps look similar.

Why the fixed ratio matters

With a single-peptide vial, the only thing that changes your draw is the water you add. With a blend, the manufacturer has already fixed the ratio between compounds before you ever add water — the 10/10 split in a Wolverine vial isn't something the calculator or the reconstitution step can adjust. If a research protocol calls for a different ratio between BPC-157 and TB-500 than the vial provides, that's a reason to reconstitute separate single-peptide vials instead of a pre-mixed blend, not to try to compensate with water volume. Water volume only ever changes concentration, never the underlying ratio.

This is also where dosing errors tend to happen with any peptide, blended or not: not at the injection, but at the reconstitution step, where the water volume added sets the concentration for every draw that follows. Getting that one number wrong carries through to every subsequent dose.

Evidence level

BPC-157 and TB-500 are each studied in preclinical research — largely animal models and in-vitro work — rather than large controlled human trials. Neither is FDA-approved for human use, and combining them into a blend doesn't change that evidence picture for either component. Treat Wolverine, like its component peptides, as a research compound rather than a proven human therapeutic.

Common mistakes

• Treating the blend's total mcg as if it were one peptide's dose. A "500 mcg Wolverine dose" is 500 mcg of combined peptide split 1:1, not 500 mcg of each component.
• Confusing mg and mcg. The vial label reads in milligrams, but Wolverine doses are typically referenced in micrograms. Recheck which unit a target dose is written in before drawing.
• Shaking instead of swirling. Inject the bacteriostatic water down the inside wall of the vial and swirl gently to dissolve.
• Losing track of which vial is which concentration. If a Wolverine vial sits in the same fridge as single-peptide BPC-157 or TB-500 vials, label each one before they get mixed up.

Related

For the reconstitution basics that apply to any peptide vial, see how to reconstitute peptides. For the math behind each individual component, see the BPC-157 dosage chart and TB-500 dosage calculator guides, or the three-peptide GLOW peptide blend dosage guide for a related blend that adds GHK-Cu. Or skip straight to the free peptide calculator and enter your Wolverine vial's total mg, water volume, and target dose directly.