How much bacteriostatic water does a GLOW peptide blend vial need?

A common research setup uses 3 mL of bacteriostatic water for a 70 mg GLOW vial (50 mg GHK-Cu, 10 mg BPC-157, 10 mg TB-500), which works out to about 23.3 mg/mL. Enter your own vial total and water volume into the calculator to see the exact concentration.

Do I reconstitute a GLOW blend differently than a single peptide?

No. The steps are the same — inject bacteriostatic water down the inside wall of the vial and swirl gently, never shake. The only difference is that a GLOW vial's total mg is split across three peptides in a fixed ratio, which affects the dose math but not the physical reconstitution process.

How does the calculator handle a GLOW blend's three peptides?

Enter the vial's total mg (for example 70 mg), the water volume, and the target total dose. The calculator returns the syringe units for that total draw. It does not split the reading into GHK-Cu, BPC-157, and TB-500 individually — those component amounts scale with the vial's fixed ratio, covered in the GLOW peptide blend dosage guide.

Can I use reverse mode to find a rounder unit reading for a GLOW blend?

Yes. Enter the vial's total mg and your target dose, and reverse mode returns the water volume needed to land that dose on a specific unit mark, the same way it works for a single-peptide vial.

GLOW Peptide Blend Reconstitution Calculator: Water & Units

By PeptCalc — Medically reviewed by David Mansour, MD — Last reviewed July 15, 2026

GLOW is a research peptide blend supplied as a single lyophilized vial containing GHK-Cu, BPC-157, and TB-500. Because all three peptides ship premixed, reconstituting the vial is a one-time step that sets the concentration for every peptide in it at once. This reference walks through that process and how to use the peptide reconstitution calculator to convert a GLOW vial and target dose into syringe units. It is written for research purposes only and is not medical advice.

What the calculator does

The peptide reconstitution calculator handles the math in two directions:

Forward mode — enter the vial's total mg, water volume, and target dose → it returns the syringe units to draw.
Reverse mode — enter the vial's total mg and target dose, plus the units you want to land on → it tells you how much water to add.

Both modes work from the same formula: concentration equals total mass divided by water volume, and units drawn equal volume in mL multiplied by 100 on a U-100 insulin syringe. For a GLOW vial, "total mg" means the combined mass of all three peptides, since that's what a single draw pulls out together.

GLOW vial composition

A GLOW vial is commonly supplied at a fixed 50 mg / 10 mg / 10 mg split:

Peptide	Amount
GHK-Cu	50 mg
BPC-157	10 mg
TB-500	10 mg
Total	70 mg

That's a 5:1:1 ratio between the three. Reconstituting the vial dissolves all three into one solution — there's no way to draw one component on its own from a premixed vial.

Typical reconstitution setup

A common research setup for a 70 mg GLOW vial uses 3 mL of bacteriostatic water:

Vial content	Water added	Concentration
70 mg (50/10/10 GLOW)	3 mL	≈23.3 mg/mL

More water lowers the concentration and produces a larger, easier-to-read draw. Less water raises the concentration and shrinks the draw. Enter a different vial total or water volume into the calculator if your GLOW vial isn't the 70 mg standard.

Reconstitution process

Gather supplies — the GLOW vial, bacteriostatic water, U-100 insulin syringes, alcohol swabs.
Swab both vial stoppers with alcohol.
Draw the calculated water volume (e.g. 3 mL for a 70 mg vial).
Inject slowly down the inside wall of the vial, not directly onto the powder.
Swirl gently — do not shake. Let it dissolve completely.
Verify the concentration matches what you entered in the calculator before drawing a dose.

For the general reconstitution steps that apply to any peptide vial, see How to Reconstitute Peptides.

The math

Step 1: Calculate concentration

Concentration = total vial mass ÷ water volume

70 mg ÷ 3 mL ≈ 23.3 mg/mL

Step 2: Convert dose to volume

Volume (mL) = target dose (mg) ÷ concentration (mg/mL)

Example: 2 mg dose ÷ 23.3 mg/mL ≈ 0.086 mL

Step 3: Convert volume to syringe units

On a U-100 insulin syringe, 1 mL equals 100 units, so:

Units = volume (mL) × 100

Example: 0.086 mL × 100 ≈ 8.6 units

The calculator performs these three steps instantly for any vial total, water volume, and dose you enter.

Worked example

A 70 mg GLOW vial (50 mg GHK-Cu, 10 mg BPC-157, 10 mg TB-500) reconstituted with 3 mL of bacteriostatic water sits at about 23.3 mg/mL. A 2 mg total dose from that vial is roughly 8.6 units on a U-100 syringe. If 8.6 units is awkward to read on your syringe, use reverse mode: enter the 70 mg vial total and 2 mg dose, set a target of 10 units, and the calculator returns the water volume that lands the draw there instead of 3 mL.

Storage

Reconstituted GLOW vials are generally kept refrigerated and protected from light, the same as any reconstituted peptide solution. Unreconstituted lyophilized powder is more stable than the mixed solution, which is why the vial ships dry. Track the date you added water so you know how long a given vial has been mixed.

Common mistakes

Treating the vial's total mg as a single peptide's dose. A "2 mg GLOW dose" is 2 mg of combined peptide across three compounds, not 2 mg of each.
Reusing a water volume from a single-peptide guide. GHK-Cu alone and a GLOW vial hold different total mg, so the same water volume produces a different concentration in each.
Shaking instead of swirling, which can degrade the peptides.
Losing track of which vial is which concentration if a GLOW vial sits in the fridge next to single-peptide vials. Label each one.

Use the calculator

Enter your GLOW vial's total mg, bacteriostatic water volume, and target dose into the peptide reconstitution calculator to see the exact syringe units, or switch to reverse mode to find the water volume that lands a dose on a specific unit mark. For the component-level breakdown of a GLOW dose across GHK-Cu, BPC-157, and TB-500, see the GLOW peptide blend dosage guide. For the reconstitution math behind each individual peptide, see the GHK-Cu reconstitution calculator, BPC-157 dosage calculator, and TB-500 dosage calculator guides.