TB-500 Units Per mL Calculator

TB-500 is the common research name for a synthetic peptide corresponding to a fragment of Thymosin Beta-4 (Tβ4), supplied as lyophilized powder for research use. A TB-500 units per mL calculator sounds like it should return a single number, but the honest answer is that units per mL never actually changes — a U-100 insulin syringe is built to read 100 units per mL no matter what's inside it. This is a research reference for what actually shifts when you reconstitute a vial: mg per unit, draw volume, and how many units a given dose takes. It is written for research purposes only and is not medical advice.

What "units per mL" actually means

Every U-100 insulin syringe divides 1 mL into 100 units. That scale is printed on the barrel and is a property of the syringe, not the peptide inside it. Someone searching "TB-500 units per mL calculator" usually isn't asking about the syringe scale itself — they're asking a different question in disguise: given how I mixed this vial, how much TB-500 is in each unit, and how many units does my target dose take?

What changes: mg per unit

Concentration is vial mass divided by water volume. Once you know mg/mL, dividing by 100 gives mg per unit — the figure that actually drives your draw.

For a 10 mg TB-500 vial:

Water added	Concentration	mg per unit	Units per mL
0.5 mL	20 mg/mL	0.2 mg/unit	100
1 mL	10 mg/mL	0.1 mg/unit	100
2 mL	5 mg/mL	0.05 mg/unit	100

Units per mL never leaves 100. Mg per unit drops as water volume rises, which is the entire lever you're pulling when you choose a reconstitution concentration.

Worked example

10 mg vial + 1 mL bacteriostatic water → 10 mg/mL

• mg per unit: 10 ÷ 100 = 0.1 mg/unit
• 2 mg dose ÷ 0.1 mg/unit = 20 units
• 5 mg dose ÷ 0.1 mg/unit = 50 units

10 mg vial + 2 mL bacteriostatic water → 5 mg/mL

• mg per unit: 5 ÷ 100 = 0.05 mg/unit
• 2 mg dose ÷ 0.05 mg/unit = 40 units
• 5 mg dose ÷ 0.05 mg/unit = 100 units

Same 2 mg dose, same syringe, two different unit readings — 20 units versus 40 — because the vials were mixed at different concentrations. Notice the second row: a 5 mg dose at 5 mg/mL draws the syringe's entire 100-unit capacity, which is one reason a more concentrated mix is often the easier one to work with. Enter your own vial size and water volume into the peptide dosage calculator to skip this arithmetic, or use reverse mode to find the water volume that lands your usual dose on a round number.

Total doses per vial doesn't change

Water volume changes the units per dose. It does not change how much TB-500 is in the vial or how many doses that vial holds. A 10 mg vial at a 2 mg dose provides 5 doses whether it was reconstituted in 0.5 mL, 1 mL, or 2 mL — only the draw size per dose shifts:

Water added	Units for a 2 mg dose	Doses in the vial
0.5 mL	10 units	5
1 mL	20 units	5
2 mL	40 units	5

Choosing a concentration for a clean reading

None of the rows above is wrong — 10 mg of TB-500 splits into 5 doses either way. What differs is how easy the draw is to read and double-check. A dose that lands on 20 units is generally easier to draw accurately on a U-100 syringe than one that lands on 3 units or 87 units, since small measurement errors matter proportionally more at the low end, and it's easier to avoid drawing right up against the syringe's 100-unit ceiling.

Reconstitution steps

1. Gather the TB-500 vial, bacteriostatic water, alcohol swabs, and a U-100 insulin syringe.
2. Swab both vial stoppers with alcohol.
3. Draw the water volume your chosen concentration calls for.
4. Inject it slowly down the inside wall of the vial, not directly onto the powder.
5. Swirl gently until fully dissolved — don't shake.
6. Confirm the resulting concentration matches what you entered into the calculator before drawing a dose.

For the general steps that apply to any peptide vial, see how to reconstitute peptides.

Common mistakes

• Treating "units per mL" as if it varies by peptide or concentration. It doesn't — it's fixed at 100 on a U-100 syringe. The figure that actually varies is mg per unit.
• Confusing TB-500's mg-scale dosing with mcg-scale peptides. TB-500 doses are typically described in milligrams, not micrograms, unlike some other repair peptides. Mixing up the two units produces a dose that's off by a factor of 1000.
• Reusing a mg-per-unit figure across a different water volume. Change the water, and the mg-per-unit figure has to be recalculated — it doesn't carry over from a previous mix.
• Confusing a smaller unit count with a smaller dose. In the total-doses table above, 10 units and 20 units both deliver the same 2 mg dose — the unit count only reflects the concentration used, not the amount of TB-500 delivered.
• Using a syringe that isn't U-100. Every calculation here assumes 100 units = 1 mL. A different syringe scale changes the unit conversion.

Use the calculator

Enter your TB-500 vial size and water volume into the peptide calculator to get concentration, mg per unit, and the exact syringe units for any target dose. Reverse mode runs it the other way — enter your dose and the units you want to land on, and it returns the water volume that gets you there.

This TB-500 units per mL breakdown and the ratio-based version in the TB-500 reconstitution ratio calculator are two views of the same math — pick whichever framing makes your draw easier to double-check. For the mcg-scale version of this question, see the BPC-157 units per mL calculator. The calculator handles the arithmetic; this guide explains what the units on the syringe actually represent.