How many units is a 25 mg NAD+ dose?

With a 500 mg NAD+ vial reconstituted in 5 mL of bacteriostatic water (100 mg/mL), a 25 mg dose works out to 25 divided by 1 mg per unit, which is 25 units on a U-100 insulin syringe.

How much bacteriostatic water goes into a NAD+ vial?

It depends on the concentration you want. A common reference point is 5 mL for a 500 mg vial, which gives 100 mg/mL and keeps typical research doses within a single syringe draw. Use the calculator to test other volumes and find one that lands your dose on a round syringe mark.

Is NAD+ injection FDA-approved?

No. NAD+ sold for research or injection use is not FDA-approved as a drug or supplement for anti-aging, performance, or wellness purposes. None of the figures in an NAD+ injection dosage calculator describe an approved or recommended human dose.

Does an NAD+ injection dosage calculator handle both mg and mcg?

Yes. Vial labels are almost always in milligrams, and because NAD+ doses run much higher than a typical peptide's, most research figures stay in milligrams throughout. 1 mg equals 1000 mcg, and the calculator converts between the two automatically if a protocol is written in mcg.

NAD+ Injection Dosage Calculator: Water & Syringe Math

By PeptCalc Research — Medically reviewed by David Mansour, MD — Last reviewed August 12, 2026

NAD+ vials are labeled in milligrams, and getting from that label to an exact syringe draw is the arithmetic an NAD+ injection dosage calculator is built to handle. This is a reference for that math: how vial size and bacteriostatic water volume set concentration, and how concentration turns a target dose into an exact draw on a U-100 insulin syringe. It is written for research purposes only and is not medical advice.

What the calculator does

An NAD+ injection dosage calculator only needs three numbers to do its job: vial size, water volume, and target dose. The peptide calculator handles the conversion in two directions:

Forward mode — enter vial size, water volume, and target dose, and it returns the syringe units to draw.
Reverse mode — enter vial size, target dose, and a desired unit count, and it returns the water volume that produces it.

Both modes run on the same formula: concentration equals mass divided by water volume, and units equal that volume in mL multiplied by 100 on a U-100 syringe. That formula doesn't change because the vial holds NAD+ instead of a peptide — only the vial's own mass and water volume matter, and NAD+ vials tend to hold far more milligrams than a typical peptide vial does.

NAD+ at a glance

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every living cell, where it drives the redox reactions cells use to convert nutrients into usable energy. It has been researched in the context of cellular energy metabolism and how NAD+ levels change with age. Much of that research base is still developing, and human trial data on injected or infused NAD+ is earlier-stage than the compound's popularity in wellness settings might suggest — a case where broad interest has outpaced the strength of the underlying human evidence.

NAD+ sold for research or injection use is not FDA-approved as a drug or supplement. It is sold as a research compound, not an approved therapy, and nothing here describes a use recommendation. It describes reconstitution arithmetic, which is identical regardless of a compound's regulatory status or how established its research is.

Typical research concentration

NAD+ most commonly reaches researchers as lyophilized powder in vials in the 500 mg to 1000 mg range — considerably larger than a typical peptide vial, because research doses of NAD+ are themselves measured in tens or hundreds of milligrams rather than micrograms. Reconstituted in 5 mL of bacteriostatic water, a reference point chosen because it keeps common research doses within a single syringe draw:

Vial size	Water added	Concentration
500 mg	5 mL	100 mg/mL
750 mg	5 mL	150 mg/mL
1000 mg	5 mL	200 mg/mL

These are reference points, not requirements — the peptide reconstitution calculator recalculates concentration for any vial size and water volume actually on hand.

The math

Step 1: Calculate concentration

Concentration = vial mass ÷ water volume

500 mg vial + 5 mL water = 500 ÷ 5 = 100 mg/mL

Step 2: Convert concentration to mg per unit

A U-100 insulin syringe divides 1 mL into 100 units, so:

mg per unit = concentration (mg/mL) ÷ 100

100 mg/mL ÷ 100 = 1 mg per unit

Step 3: Convert the target dose to units

Units = target dose (mg) ÷ mg per unit

Worked example. A 500 mg NAD+ vial reconstituted in 5 mL of bacteriostatic water is 100 mg/mL, which is 1 mg per unit. A 25 mg research reference dose from that vial is 25 ÷ 1 = 25 units. A 50 mg dose from the same vial is 50 ÷ 1 = 50 units.

Compare that to the same 500 mg vial reconstituted in just 2 mL of water instead: 500 ÷ 2 = 250 mg/mL, which is 2.5 mg per unit. That same 50 mg dose is now 50 ÷ 2.5 = 20 units — a smaller draw from a higher-concentration vial, even though the dose in mg hasn't changed at all.

Choosing a water volume

Water volume is the lever that sets how large a given dose reads on the syringe. For a 500 mg vial and a 50 mg dose:

Water added	Concentration	mg per unit	Units for 50 mg
2 mL	250 mg/mL	2.5 mg/unit	20 units
5 mL	100 mg/mL	1 mg/unit	50 units
10 mL	50 mg/mL	0.5 mg/unit	100 units

None of these rows is more correct than another — 50 mg is 50 mg regardless of which row it comes from. A U-100 syringe caps out at 100 units per mL, so a low-concentration mix that pushes a single dose to the top of the barrel leaves no room to round up; a higher concentration keeps the draw smaller and leaves more headroom. Reverse mode on the peptide dosage calculator finds the water volume that lands a specific dose on a round unit mark.

Reconstitution process

Gather the NAD+ vial, bacteriostatic water, a U-100 insulin syringe, and alcohol swabs.
Swab both vial stoppers with alcohol.
Draw the water volume the chosen concentration calls for.
Inject it slowly down the inside wall of the vial, not directly onto the powder.
Swirl gently until fully dissolved — don't shake, and don't be surprised if it takes longer to dissolve than a smaller peptide vial does, given the larger mass involved.
Confirm the resulting concentration matches what was entered into the calculator before drawing a dose.

For the general mixing walkthrough that applies to any peptide or research-compound vial, see how to reconstitute peptides.

Storage

Reconstituted NAD+ is generally kept refrigerated and protected from light, since NAD+ is a light- and temperature-sensitive molecule. Unreconstituted lyophilized powder is far more stable than the mixed solution, which is why vials ship dry. Track the date each vial was reconstituted, particularly if more than one vial is in use at different concentrations.

Common mistakes

Assuming NAD+ doses look like peptide doses. Most peptides are dosed in micrograms; NAD+ research doses typically run in the tens to hundreds of milligrams. Applying a peptide-scale mental model to NAD+ produces a dramatically wrong unit count.
Treating research figures as established human dosing. NAD+'s human research base is still developing. A number appearing in a research protocol is not the same as a validated clinical dose.
Reusing a unit count across a different water volume. As the table above shows, 50 mg is 20 units at one concentration and 100 units at another. A unit count only means something relative to the specific concentration it was calculated from.
Using a syringe that isn't U-100. Every unit conversion here assumes 100 units equal 1 mL. A different syringe scale changes the math entirely.
Skipping a fresh concentration check after reconstitution. Confirm the math before drawing, especially when switching between vial sizes.

Used as an NAD+ injection dosage calculator, the free peptide calculator skips the arithmetic and returns exact syringe units, with reverse mode available for finding a water volume that lands a dose on a clean mark. For the general reconstitution process that applies to any vial, see how to reconstitute peptides.