Melanotan 2 Reconstitution Calculator: Water & Units

Melanotan 2 (MT-2) is a synthetic peptide analog of alpha-melanocyte-stimulating hormone, supplied as lyophilized powder for research use. Like other lyophilized peptides, it has to be reconstituted with a liquid before it can be measured or drawn into a syringe. This reference explains how a Melanotan 2 reconstitution calculator turns a vial size, water volume, and target mcg dose into the exact number of units to draw on a U-100 insulin syringe. It is written for research purposes only and is not medical advice.

What the calculator does

The peptide reconstitution calculator handles the math in two directions:

• Forward mode — enter vial size, water volume, and target dose → it returns the syringe units to draw.
• Reverse mode — enter vial size, target dose, and desired syringe units → it tells you how much water to add.

Both modes run on the same formula: concentration equals mass divided by volume, and dose in units equals dose in volume times 100 on a U-100 syringe.

Common Melanotan 2 vial sizes

Research vials are typically supplied in:

• 10 mg

The calculator supports any vial size, but 10 mg is the size most often listed by research suppliers for Melanotan 2.

Typical research concentration

A common reconstitution setup for Melanotan 2 holds the water at 2 mL for a 10 mg vial:

Vial size	Water added	Concentration
10 mg	2 mL	5,000 mcg/mL

That 5,000 mcg/mL reference point puts the typical 250-500 mcg research dose range on clean unit marks:

Target dose	Units at 5,000 mcg/mL
250 mcg	5 units
500 mcg	10 units

A 250 mcg dose landing on an exact 5-unit mark is a large part of why 2 mL is a common water volume for a 10 mg Melanotan 2 vial — it reads cleanly on any U-100 syringe.

The math

Step 1: Calculate concentration

Concentration = vial mass ÷ water volume

• 10 mg vial + 2 mL water = 10,000 mcg ÷ 2 mL = 5,000 mcg/mL
• 10 mg vial + 1 mL water = 10,000 mcg ÷ 1 mL = 10,000 mcg/mL

Step 2: Convert dose to volume

Volume (mL) = dose (mcg) ÷ concentration (mcg/mL)

Example: 250 mcg dose at 5,000 mcg/mL concentration = 250 ÷ 5,000 = 0.05 mL

Step 3: Convert volume to syringe units

On a U-100 insulin syringe, 1 mL = 100 units, so:

Units = volume (mL) × 100

Example: 0.05 mL × 100 = 5 units

The calculator performs these three steps instantly for any vial, water, and dose combination you enter.

Reconstitution process

1. Gather supplies — the Melanotan 2 vial, bacteriostatic water, U-100 insulin syringes, alcohol swabs.
2. Swab both vial stoppers with alcohol.
3. Draw the calculated water volume (e.g. 2 mL for a 10 mg vial at 5,000 mcg/mL).
4. Inject slowly down the inside wall of the vial, not directly onto the powder.
5. Swirl gently — do not shake. Let it dissolve completely.
6. Verify the concentration matches what you entered in the calculator.

For the general reconstitution steps that apply to any peptide vial, see How to Reconstitute Peptides.

Storage

Reconstituted peptide vials are generally stored refrigerated and protected from light. Unreconstituted lyophilized powder is stable at room temperature until the expiration date on the vial. Track the reconstitution date so you know how long a given vial has been mixed.

Why the water volume matters

The same 250 mcg dose can be drawn at very different unit marks depending on how much water goes into the vial:

250 mcg dose from a 10 mg vial:

Water added	Concentration	Draw volume	Units
2 mL	5,000 mcg/mL	0.05 mL	5 units
1 mL	10,000 mcg/mL	0.025 mL	2.5 units

Neither is "wrong" — the math is identical either way. A 2.5-unit reading only works cleanly on a syringe marked in half-unit increments, which is part of why 2 mL is the more commonly used water volume for Melanotan 2. The calculator shows the units for any water volume so you can pick the draw size that's easiest to read on your syringe.

Common mistakes

• Confusing mg and mcg. Melanotan 2 vials are labeled in milligrams, but doses are almost always described in micrograms. A 10 mg vial is 10,000 mcg — mixing up the two units produces a tenfold dosing error.
• Shaking the vial. Swirl gently instead — shaking can degrade the peptide.
• Not recalculating after changing the water volume. Adding a different amount of bacteriostatic water than the last vial changes the concentration, so the same unit mark no longer matches the same dose. Recalculate for each vial rather than reusing a number from memory.
• Using a non-U-100 syringe. The calculator assumes a standard U-100 insulin syringe (100 units = 1 mL). If your syringe is marked differently, the unit conversion will not match.

Use the calculator

Enter your Melanotan 2 vial size, bacteriostatic water volume, and target dose into the peptide reconstitution calculator to see the exact syringe units. Reverse mode also works — enter your target dose and the units you want to draw, and it tells you how much water to add.

For related math, see the MT-2 dosage calculator guide, the how to reconstitute peptides walkthrough, or the TB-500 dosage calculator and GHK-Cu reconstitution calculator guides for peptides measured in milligrams instead of micrograms. The calculator handles the math. This guide explains what the numbers mean.