How many units is a 100 mcg DSIP dose?

With a 5 mg DSIP vial reconstituted in 2 mL of bacteriostatic water (2500 mcg/mL), a 100 mcg dose works out to 100 divided by 25 mcg per unit, which is 4 units on a U-100 insulin syringe.

How much bacteriostatic water goes into a 5 mg DSIP vial?

It depends on the concentration you want. A common reference point is 2 mL, which gives 2500 mcg/mL and keeps typical research doses in a readable unit range. Use the calculator to test other volumes and find one that lands your dose on a round syringe mark.

Is DSIP FDA-approved?

No. DSIP is not FDA-approved for any use. It is sold as a research compound, not as a drug or supplement, and none of the figures in a DSIP dosage calculator describe an approved or recommended human dose.

Does a DSIP dosage calculator handle both mg and mcg?

Yes. Vial labels are almost always in milligrams while research doses are typically described in micrograms. 1 mg equals 1000 mcg, and the calculator converts between the two automatically once a vial size, water volume, and target dose are entered.

DSIP Dosage Calculator: Reconstitution & Syringe Units

By PeptCalc Research — Medically reviewed by David Mansour, MD — Last reviewed August 11, 2026

DSIP vials are labeled in milligrams, while research doses are typically described in micrograms — the exact conversion a DSIP dosage calculator is built to handle. This is a reference for the arithmetic behind that vial: how vial size and bacteriostatic water volume set concentration, and how concentration turns a target dose into an exact draw on a U-100 insulin syringe. It is written for research purposes only and is not medical advice.

What the calculator does

A DSIP dosage calculator only needs three numbers to do its job: vial size, water volume, and target dose. The peptide dosage calculator handles the conversion in two directions:

Forward mode — enter vial size, water volume, and target dose, and it returns the syringe units to draw.
Reverse mode — enter vial size, target dose, and a desired unit count, and it returns the water volume that produces it.

Both modes run on the same formula: concentration equals mass divided by water volume, and units equal that volume in mL multiplied by 100 on a U-100 syringe. That formula doesn't change because the vial holds DSIP instead of another peptide — only the vial's own mass and water volume matter.

DSIP at a glance

DSIP, short for delta sleep-inducing peptide, is a nonapeptide first described in early sleep-architecture research and studied for a possible role in regulating slow-wave ("delta") sleep. Later research has also looked at its relationship to the body's stress-response signaling. Human data is limited, and results across the small trials that exist have been mixed rather than conclusive — a peptide where the research base is thinner than its name suggests.

DSIP is not FDA-approved for any use. It is sold as a lyophilized research compound, not as a drug or supplement, and nothing here describes a use recommendation. It describes reconstitution arithmetic, which is identical regardless of a compound's regulatory status or how well-established its research is.

Typical research concentration

DSIP most commonly reaches researchers as lyophilized powder in vials in the 2 mg to 10 mg range. Reconstituted in 2 mL of bacteriostatic water, a reference point chosen because it keeps typical research doses on readable syringe marks:

Vial size	Water added	Concentration
2 mg	2 mL	1 mg/mL (1000 mcg/mL)
5 mg	2 mL	2.5 mg/mL (2500 mcg/mL)
10 mg	2 mL	5 mg/mL (5000 mcg/mL)

These are reference points, not requirements — the peptide calculator recalculates concentration for any vial size and water volume actually on hand.

The math

Step 1: Calculate concentration

Concentration = vial mass ÷ water volume

5 mg vial + 2 mL water = 5 ÷ 2 = 2.5 mg/mL (2500 mcg/mL)

Step 2: Convert concentration to mcg per unit

A U-100 insulin syringe divides 1 mL into 100 units, so:

mcg per unit = concentration (mcg/mL) ÷ 100

2500 mcg/mL ÷ 100 = 25 mcg per unit

Step 3: Convert the target dose to units

Units = target dose (mcg) ÷ mcg per unit

Worked example. A 5 mg DSIP vial reconstituted in 2 mL of bacteriostatic water is 2500 mcg/mL, which is 25 mcg per unit. A 100 mcg research reference dose from that vial is 100 ÷ 25 = 4 units. A 250 mcg dose from the same vial is 250 ÷ 25 = 10 units.

Compare that to a 10 mg vial in the same 2 mL of water: 10 ÷ 2 = 5 mg/mL, or 5000 mcg/mL, which is 50 mcg per unit. That same 100 mcg dose is now 100 ÷ 50 = 2 units — a smaller draw from a higher-concentration vial, even though the dose in mcg hasn't changed at all.

Choosing a water volume

Water volume is the lever that sets how large a given dose reads on the syringe. For a 5 mg vial and a 100 mcg dose:

Water added	Concentration	mcg per unit	Units for 100 mcg
1 mL	5000 mcg/mL	50 mcg/unit	2 units
2 mL	2500 mcg/mL	25 mcg/unit	4 units
5 mL	1000 mcg/mL	10 mcg/unit	10 units

None of these rows is more correct than another — 100 mcg is 100 mcg regardless of which row it comes from. A larger draw is generally easier to measure precisely on a U-100 barrel than a very small one, since a one-unit misread is a bigger proportional error at the low end. Reverse mode on the free peptide calculator finds the water volume that lands a specific dose on a round unit mark.

Reconstitution process

Gather the DSIP vial, bacteriostatic water, a U-100 insulin syringe, and alcohol swabs.
Swab both vial stoppers with alcohol.
Draw the water volume the chosen concentration calls for.
Inject it slowly down the inside wall of the vial, not directly onto the powder.
Swirl gently until fully dissolved — don't shake.
Confirm the resulting concentration matches what was entered into the calculator before drawing a dose.

For the general mixing walkthrough that applies to any peptide vial, see how to reconstitute peptides.

Storage

Reconstituted DSIP is generally kept refrigerated and protected from light. Unreconstituted lyophilized powder is far more stable than the mixed solution, which is why vials ship dry. Track the date each vial was reconstituted, particularly if more than one vial is in use at different concentrations.

Common mistakes

Confusing mg and mcg. DSIP vial labels are in milligrams; research doses are typically described in micrograms. 1 mg equals 1000 mcg, and misreading one for the other produces a 1000x error.
Treating research figures as established human dosing. DSIP's human research base is small and its findings are mixed. A number appearing in a research protocol is not the same as a validated clinical dose.
Reusing a unit count across a different water volume. As the table above shows, 100 mcg is 4 units at one concentration and 2 units at another. A unit count only means something relative to the specific concentration it was calculated from.
Using a syringe that isn't U-100. Every unit conversion here assumes 100 units equal 1 mL. A different syringe scale changes the math entirely.
Skipping a fresh concentration check after reconstitution. Confirm the math before drawing, especially when switching between vial sizes.

Used as a DSIP dosage calculator, the peptide calculator skips the arithmetic and returns exact syringe units, with reverse mode available for finding a water volume that lands a dose on a clean mark. For the general reconstitution process that applies to any vial, see how to reconstitute peptides.