How many units is a 250 mcg AOD-9604 dose?

With a 5 mg AOD-9604 vial reconstituted in 2 mL of bacteriostatic water (2.5 mg/mL, or 2500 mcg/mL), a 250 mcg dose works out to 250 divided by 25 mcg per unit, which is 10 units on a U-100 insulin syringe.

How much bacteriostatic water goes into an AOD-9604 vial?

It depends on the concentration you want. A common reference point is 2 mL for a 5 mg vial, which gives 2.5 mg/mL and keeps typical research doses in a readable unit range. Use the calculator to test other volumes and find one that lands your dose on a round syringe mark.

Is AOD-9604 FDA-approved?

No. AOD-9604 is not FDA-approved for any use. It is sold as a research compound, not as a drug or supplement, and none of the figures in an AOD-9604 dosage calculator describe an approved or recommended human dose.

Does an AOD-9604 dosage calculator handle both mg and mcg?

Yes. Vial labels are almost always in milligrams while research protocols often describe doses in micrograms. 1 mg equals 1000 mcg, and the calculator converts between the two automatically once a vial size, water volume, and target dose are entered.

AOD-9604 Dosage Calculator: Reconstitution & Syringe Units

By PeptCalc Research — Medically reviewed by David Mansour, MD — Last reviewed August 13, 2026

AOD-9604 vials are labeled in milligrams, while research doses are usually written in micrograms — the exact conversion an AOD-9604 dosage calculator is built to handle. This is a reference for the arithmetic behind that vial: how vial size and bacteriostatic water volume set concentration, and how concentration turns a target dose into an exact draw on a U-100 insulin syringe. It is written for research purposes only and is not medical advice.

What the calculator does

An AOD-9604 dosage calculator only needs three numbers to do its job: vial size, water volume, and target dose. The peptide calculator handles the conversion in two directions:

Forward mode — enter vial size, water volume, and target dose, and it returns the syringe units to draw.
Reverse mode — enter vial size, target dose, and a desired unit count, and it returns the water volume that produces it.

Both modes run on the same formula: concentration equals mass divided by water volume, and units equal that volume in mL multiplied by 100 on a U-100 syringe. That formula doesn't change because the vial holds AOD-9604 instead of another peptide — only the vial's own mass and water volume matter.

AOD-9604 at a glance

AOD-9604 is a modified synthetic fragment corresponding to the C-terminal region of human growth hormone, commonly referenced as hGH fragment 176-191. It was developed to isolate the region of the hGH molecule associated with fat metabolism from the region associated with growth and blood-sugar effects, and it has been studied in that context rather than as a growth-hormone replacement.

AOD-9604 is not FDA-approved for any use. It is sold as a lyophilized research compound, not as a drug or supplement, and nothing here describes a use recommendation. It describes reconstitution arithmetic, which is identical regardless of a compound's regulatory status or how far along its research is.

Typical research concentration

AOD-9604 most commonly reaches researchers as lyophilized powder in vials in the 2 mg to 5 mg range. Reconstituted in 2 mL of bacteriostatic water, a reference point chosen because it keeps typical research doses on readable syringe marks:

Vial size	Water added	Concentration
2 mg	2 mL	1 mg/mL (1000 mcg/mL)
5 mg	2 mL	2.5 mg/mL (2500 mcg/mL)
10 mg	2 mL	5 mg/mL (5000 mcg/mL)

These are reference points, not requirements — the peptide reconstitution calculator recalculates concentration for any vial size and water volume actually on hand.

The math

Step 1: Calculate concentration

Concentration = vial mass ÷ water volume

5 mg vial + 2 mL water = 5 ÷ 2 = 2.5 mg/mL (2500 mcg/mL)

Step 2: Convert concentration to mcg per unit

A U-100 insulin syringe divides 1 mL into 100 units, so:

mcg per unit = concentration (mcg/mL) ÷ 100

2500 mcg/mL ÷ 100 = 25 mcg per unit

Step 3: Convert the target dose to units

Units = target dose (mcg) ÷ mcg per unit

Worked example. A 5 mg AOD-9604 vial reconstituted in 2 mL of bacteriostatic water is 2.5 mg/mL, or 2500 mcg/mL, which is 25 mcg per unit. A 250 mcg research reference dose from that vial is 250 ÷ 25 = 10 units. A 500 mcg dose from the same vial is 500 ÷ 25 = 20 units.

Compare that to a 2 mg vial in the same 2 mL of water: 2 ÷ 2 = 1 mg/mL, or 1000 mcg/mL, which is 10 mcg per unit. That same 250 mcg dose is now 250 ÷ 10 = 25 units — a larger draw from a lower-concentration vial, even though the dose in mcg hasn't changed at all.

Choosing a water volume

Water volume is the lever that sets how large a given dose reads on the syringe. For a 5 mg vial and a 250 mcg dose:

Water added	Concentration	mcg per unit	Units for 250 mcg
1 mL	5000 mcg/mL	50 mcg/unit	5 units
2 mL	2500 mcg/mL	25 mcg/unit	10 units
5 mL	1000 mcg/mL	10 mcg/unit	25 units

None of these rows is more correct than another — 250 mcg is 250 mcg regardless of which row it comes from. A larger draw is generally easier to measure precisely on a U-100 barrel than a very small one, since a one-unit misread is a bigger proportional error at the low end. Reverse mode on the free peptide calculator finds the water volume that lands a specific dose on a round unit mark.

Reconstitution process

Gather the AOD-9604 vial, bacteriostatic water, a U-100 insulin syringe, and alcohol swabs.
Swab both vial stoppers with alcohol.
Draw the water volume the chosen concentration calls for.
Inject it slowly down the inside wall of the vial, not directly onto the powder.
Swirl gently until fully dissolved — don't shake.
Confirm the resulting concentration matches what was entered into the calculator before drawing a dose.

For the general mixing walkthrough that applies to any peptide vial, see how to reconstitute peptides.

Storage

Reconstituted AOD-9604 is generally kept refrigerated and protected from light. Unreconstituted lyophilized powder is far more stable than the mixed solution, which is why vials ship dry. Track the date each vial was reconstituted, particularly if more than one vial is in use at different concentrations.

Common mistakes

Confusing mg and mcg. AOD-9604 vial labels are in milligrams; research doses are almost always described in micrograms. 1 mg equals 1000 mcg, and misreading one for the other produces a 1000x error.
Mistaking AOD-9604 for full-length growth hormone. AOD-9604 is a small fragment of the hGH molecule, studied for a narrower purpose than the intact hormone, and the two are not interchangeable in a research protocol.
Treating research figures as established human dosing. A number appearing in a research protocol is not the same as a validated clinical dose, particularly for a compound with no FDA-approved use.
Reusing a unit count across a different water volume. As the table above shows, 250 mcg is 10 units at one concentration and 25 units at another. A unit count only means something relative to the specific concentration it was calculated from.
Using a syringe that isn't U-100. Every unit conversion here assumes 100 units equal 1 mL. A different syringe scale changes the math entirely.

Used as an AOD-9604 dosage calculator, the peptide calculator skips the arithmetic and returns exact syringe units, with reverse mode available for finding a water volume that lands a dose on a clean mark. For the general reconstitution process that applies to any vial, see how to reconstitute peptides.