How much bacteriostatic water goes into a 5-Amino-1MQ vial?

It depends on the concentration you want. A common reference point is 2 mL of bacteriostatic water for a 50 mg vial, giving 25 mg/mL. Use the calculator to test other water volumes and find one that lands your dose on a clean unit mark.

How many units is a 5 mg 5-Amino-1MQ dose?

At 25 mg/mL (a 50 mg vial reconstituted in 2 mL of bacteriostatic water), 25 mg/mL divided by 100 units per mL is 0.25 mg per unit, so a 5 mg dose is 5 divided by 0.25, which is 20 units on a U-100 insulin syringe.

Is 5-Amino-1MQ FDA-approved?

No. 5-Amino-1MQ is not FDA-approved as a drug for weight management or any other condition. It's sold as a research chemical, not an approved therapy, and nothing in a 5-Amino-1MQ dosage calculator describes an approved or recommended human dose.

Is 5-Amino-1MQ a peptide?

No. It's a small molecule that inhibits the NNMT enzyme and has no amino acid backbone, unlike true peptides such as BPC-157 or TB-500. It's covered here because it ships the same way peptides do — as a lyophilized powder reconstituted with bacteriostatic water — so the same vial math applies.

5-Amino-1MQ Dosage Calculator: Vial and Syringe Math

By PeptCalc Research — Medically reviewed by David Mansour, MD — Last reviewed August 15, 2026

5-Amino-1MQ ships as a lyophilized powder in a sealed vial, and turning that vial into an exact syringe draw takes the same three-step arithmetic used for any research compound sold this way. This is a reference for that math: how vial size and bacteriostatic water volume set concentration, and how concentration converts a target 5-Amino-1MQ dose into an exact number of units on a U-100 insulin syringe. It is written for research purposes only and is not medical advice.

What the calculator does

A 5-Amino-1MQ dosage calculator only needs three inputs to do its job: vial size, water volume, and target dose. The peptide calculator handles the conversion in two directions:

Forward mode — enter vial size, water volume, and target dose, and it returns the syringe units to draw.
Reverse mode — enter vial size, target dose, and a desired unit count, and it returns the water volume that produces it.

Both modes run on the same formula: concentration equals mass divided by water volume, and units equal that volume in mL multiplied by 100 on a U-100 syringe. The formula doesn't care what's in the vial — only the numbers going in change from one compound to the next.

What 5-Amino-1MQ is

5-Amino-1MQ is a small molecule that inhibits nicotinamide N-methyltransferase (NNMT), an enzyme expressed in fat tissue that regulates methyl-group metabolism and cellular NAD+ turnover. It has no amino acid backbone, so it isn't technically a peptide, but it's sold and dosed the way most research peptides are: as a lyophilized powder that a researcher reconstitutes with bacteriostatic water before drawing a dose.

The preclinical evidence behind NNMT inhibition is animal-model work. Neelakantan and colleagues reported that a selective, membrane-permeable NNMT inhibitor — the compound now sold as 5-Amino-1MQ — reversed high-fat-diet-induced obesity in mice (Biochemical Pharmacology, 2018). That's mouse data, not a human trial, and it should be read as such. 5-Amino-1MQ is not FDA-approved as a drug for weight management or any other condition, and nothing here describes an approved or recommended human use.

Common vial sizes

Research suppliers most often list 5-Amino-1MQ powder in:

50 mg
100 mg

The calculator supports any vial size, but 50 mg is the size most researchers encounter first.

Typical research concentration

A 50 mg 5-Amino-1MQ vial lands at a different concentration depending on how much bacteriostatic water goes in:

Water added	Concentration	mg per unit
1 mL	50 mg/mL	0.5 mg/unit
2 mL	25 mg/mL	0.25 mg/unit
2.5 mL	20 mg/mL	0.2 mg/unit

These are reference points, not requirements — the peptide reconstitution calculator recalculates concentration for any vial size and water volume actually on hand.

The math

Step 1: Calculate concentration

Concentration = vial mass ÷ water volume

50 mg vial + 2 mL water = 50 ÷ 2 = 25 mg/mL

Step 2: Convert concentration to mg per unit

A U-100 insulin syringe divides 1 mL into 100 units, so:

mg per unit = concentration (mg/mL) ÷ 100

25 mg/mL ÷ 100 = 0.25 mg per unit

Step 3: Convert the target dose to units

Units = target dose (mg) ÷ mg per unit

Worked example. A 50 mg 5-Amino-1MQ vial reconstituted in 2 mL of bacteriostatic water is 25 mg/mL, which is 0.25 mg per unit. A 5 mg research reference dose from that vial is 5 ÷ 0.25 = 20 units. A 2.5 mg dose is 2.5 ÷ 0.25 = 10 units.

Compare the same 50 mg vial reconstituted in 1 mL of water instead: 50 ÷ 1 = 50 mg/mL, which is 0.5 mg per unit. That same 5 mg dose is now 5 ÷ 0.5 = 10 units — a much smaller draw from a higher-concentration vial, even though the dose in mg hasn't changed.

Why the water volume matters

For a 50 mg vial and a 5 mg dose:

Water added	Concentration	mg per unit	Units for 5 mg
1 mL	50 mg/mL	0.5 mg/unit	10 units
2 mL	25 mg/mL	0.25 mg/unit	20 units
2.5 mL	20 mg/mL	0.2 mg/unit	25 units

None of these rows is more correct than another — 5 mg is 5 mg regardless of which row it comes from. Less water concentrates the dose into a smaller draw; more water spreads it across more of the syringe barrel, which can make a small dose easier to read. The free peptide calculator checks any water volume against any target dose in seconds.

Reconstitution process

Gather the 5-Amino-1MQ vial, bacteriostatic water, a U-100 insulin syringe, and alcohol swabs.
Swab both vial stoppers with alcohol.
Draw the water volume the chosen concentration calls for.
Inject it slowly down the inside wall of the vial, not directly onto the powder.
Swirl gently until fully dissolved — don't shake.
Confirm the resulting concentration matches what was entered into the calculator before drawing a dose.

For the general mixing walkthrough that applies to any lyophilized vial, see how to reconstitute peptides.

Storage

Reconstituted 5-Amino-1MQ is generally kept refrigerated and protected from light once it's in solution. Unreconstituted lyophilized powder is far more stable than the mixed solution, which is why it ships dry rather than pre-mixed. Track the date each vial was reconstituted, and check the supplier's guidance for how long a mixed vial is meant to be used before it should be discarded.

Common mistakes

Treating 5-Amino-1MQ dosing like a microgram-scale peptide. It's dosed and vialed in milligrams, closer in scale to glutathione than to a typical microgram-dosed peptide. Applying a microgram mental model produces a badly wrong unit count.
Confusing 5 mg with 5,000 mcg and reading the label wrong. 1 mg equals 1,000 mcg, so a 50 mg vial holds 50,000 mcg. Misreading a milligram label as micrograms — or the reverse — is the fastest way to be off by three orders of magnitude.
Reusing a unit count across a different water volume. As the table above shows, 5 mg is 10 units at one concentration and 25 units at another. A unit count only means something relative to the specific concentration it was calculated from.
Using a syringe that isn't U-100. Every unit conversion here assumes 100 units equal 1 mL. A different syringe scale changes the math entirely.
Treating the mouse data as proof of a human effect. The NNMT-inhibition research behind 5-Amino-1MQ is animal-model evidence. It's a reason the compound is studied, not a guarantee of the same result in a person.

Used as a 5-Amino-1MQ dosage calculator, the peptide calculator skips the arithmetic and returns exact syringe units, with reverse mode available for finding a water volume that lands a dose on a clean mark. For the general reconstitution process that applies to any vial, see how to reconstitute peptides.